刃天青的红色代谢产物可用分光光度计(波长570nm 或 600nm)或荧光计(激发光波长在530-560 nm之间,发射光波长为590 nm)进行测定。试剂盒操作简便、快速(一步法),安全和可靠,其最低检测灵敏度可以达到40个细胞。
Kit 规格: HT29 细胞密度,10,000细胞/孔(80 μL)。将细胞用不同浓度的saponin 抗体螯合物处理 (10 ul),培养3天,加入10μl/孔细胞兰TM 溶液。总反应体系100 ul. 继续将细胞培养4小时,读取吸光值。数据平均值± SD (n = 3).
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